- 您現(xiàn)在的位置：買賣IC網(wǎng) > PDF目錄374671 > S7804 (Electronic Theatre Controls, Inc.) CpG WIZ Prader-Willi/Angelman Amplification Kit PDF資料下載
參數(shù)資料
| 型號： | S7804 |
| 廠商： | Electronic Theatre Controls, Inc. |
| 英文描述： | CpG WIZ Prader-Willi/Angelman Amplification Kit |
| 中文描述： | 中央人民政府武進(jìn)Prader-Willi/Angelman擴(kuò)增試劑盒 |
| 文件頁數(shù)： | 12/32頁 |
| 文件大?。?/td> | 386K |
| 代理商： | S7804 |
第1頁第2頁第3頁第4頁第5頁第6頁第7頁第8頁第9頁第10頁第11頁當(dāng)前第12頁第13頁第14頁第15頁第16頁第17頁第18頁第19頁第20頁第21頁第22頁第23頁第24頁第25頁第26頁第27頁第28頁第29頁第30頁第31頁第32頁
8
“Hot Start” PCR
The three sets of primers used in the CpG WIZ Methylation Assay are derived
from sequences closely related to each other, which introduces the possibility of
mispriming. In order to avoid this and other PCR-related artifacts, "hot start"
PCR is recommended. "Hot start" PCR permits the Taq polymerase to begin the
reaction only after the template and primers are in single-stranded form.
There are several modifications of the standard PCR protocol which allow a "hot
start" to occur. In one scenario, the PCR reaction mixture excluding the
polymerase can be overlaid with mineral oil prior to heating to 95°C. At the end
of the incubation, the enzyme is pipetted directly into the mixture under the
mineral oil. A second method involves the physical separation of the polymerase
and the rest of the PCR mix with a wax bead. The enzyme combines with the
rest of the reaction mixture only after the wax melts. In another variation, an
anti-Taq antibody inhibits the polymerase during reaction setup by forming a
complex with the Taq enzyme. Taq polymerase becomes active when the
complex is abolished due to antibody denaturation during the 95°C incubation.
Alternatively, a "hot start" enzyme can be used. Refer to the manufacturer's
instructions for enzyme activation protocol.
Note:
Do not use a polymerase with 3'-5' exonuclease activity (i.e.
proofreading).Do not use a wax bead that contains Mg
2+
. Any extra Mg
2+
added
to the reaction mixture produces suboptimal results.
Genomic Control DNAs
The methylated (red cap) and unmethylated (white cap) control DNAs must
undergo bisulfite modification prior to PCR amplification using the
CpGenome DNA Modification Kit (S7820). When used with their respective
U and M primer sets, a single PCR product of an expected size is obtained in
each case.
W control genomic DNA is used in the PCR and is NOT to be used in the
chemical modification step. When used with the W primer set, it is a positive
control for PCR amplification. Failure to generate a PCR product indicates a
general failure in the PCR reaction.
相關(guān)PDF資料 |
PDF描述 |
|---|---|
| S7B-EH | BZ Series Standard Basic Switch, Single Pole Double Throw Circuitry, 15 A at 250 Vac, Overtravel Plunger Actuator, 2,5 N - 3,61 N [9 oz - 13 oz] Operating Force], Silver Contacts, Screw Termination, CE, CSA |
| S8040N | SCRs (1 A to 70 A) |
| S8040N | SCRs |
| S8040R | SCRs |
| S8055M | SCRs |
相關(guān)代理商/技術(shù)參數(shù) |
參數(shù)描述 |
|---|---|
| S78-0400152-N40 | 制造商:Micro-Star International 功能描述:MSI CARD READER & WRITER - Bulk |
| S7805D | 制造商:AUK 制造商全稱:AUK corp 功能描述:Fixed Voltage Regulator |
| S7805D_06 | 制造商:AUK 制造商全稱:AUK corp 功能描述:Fixed Voltage Regulator |
| S7805P | 制造商:AUK 制造商全稱:AUK corp 功能描述:Fixed Voltage Regulator |
| S7805PI | 制造商:AUK 制造商全稱:AUK corp 功能描述:Fixed Voltage Regulator |
發(fā)布緊急采購，3分鐘左右您將得到回復(fù)。